Environment

Rovaltain Research Company

Ecotoxicology and environmental fate

The services provided in ecotoxicology and environmental fate is Rovaltain Research Company core business.

RRCo aims to offer tests according to the OECD guidelines, particularly tests from the series 200 (biological systems) in static, semi-static or dynamic version.

Thanks to laboratories with a controlled environment (hygrometry, temperature and lighting), RRCo offers assistance in your projects or studies requiring secured and adapted conditions.

It is also possible to implement a controlled environment with biosafety level 2 or 3. These environments are mainly dedicated to chronic or acute exposure experiments using potentially hazardous test substance.

Tests for which the objective is to determine the impact of a substance on marine environment, RRCo offers the possibility of adapting guidelines to sea water and selects the organisms adapted to your needs.

RRCo adapts to your requirements regulatory tests as part of R&D project.

Freshwater and sea water

Effluents – Solid or liquid substances

OECD tests by RRCo

Aquatic ecotoxicology
RRCo aims to offer tests allowing to define the ecotoxicity on aquatic organisms.

    • OECD 201 Freshwater Alga and Cyanobacteria, Growth Inhibition Test
    • OECD 202 Daphnia sp. Acute Immobilisation Test
    • OECD 203 (water and sea water) Fish, Acute Toxicity Test
    • OECD 210 Fish, Early-life Stage Toxicity Test
    • OECD 211 Daphnia magna Reproduction Test
    • OECD 212Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages
    • OECD 215 Fish, Juvenile Growth Test
    • OECD 218 Sediment-Water Chironomid Toxicity Using Spiked Sediment
    • OECD 219 Sediment-Water Chironomid Toxicity Using Spiked Waternt
    • OECD 221 Lemna sp. Growth Inhibition Test
    • OECD 236 Fish Embryo Acute Toxicity (FET) Teste
    • OECD 238  Sediment-Free Myriophyllum Spicatum Toxicity Test
    • OECD 239 Water-Sediment Myriophyllum Spicatum Toxicity Test

    All the experiments can be carried out according to GLP « Good Laboratory Practice »

    RRCo aims to offer aquatic ecotoxicological tests according to the OECD Guidelines or ISO norms.

    For effluent, pure substance, multi-constituent, UVCB or with specific physical-chemical or toxicological properties (volatility, insolubility, toxicity level 2) RRCo offers to adapt tests to a regulatory needs.

    For organic substance, RRCo offers to develop and validate analytical methods allowing test item monitoring during the various phase of test.

Terrestrial ecotoxicology

RRCo aims to offer tests allowing to define the ecotoxicity on terrestrial organisms and plants.

  • OECD 207 Earthworm, Acute Toxicity Tests
  • OECD 208 Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test
  • OECD 209 Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation)
  • OECD 216 Soil Microorganisms: Nitrogen Transformation Test
  • OECD 217 Soil Microorganisms: Carbon Transformation Test
  • OECD 222 Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei)
  • OECD 227 Terrestrial Plant Test: Vegetative Vigour Test
  • OECD 232 Collembolan Reproduction Test in Soil
  • Aphidius rhopalosiphi : Mead Briggs et al. 2000 – A laboratory test for evaluating the effects of plant protection products on the parasitic wasp, Aphidius rhopalosiphi (DeStephani-Perez) (Hymenoptera, Braconidae)*
  • Aleochara : Grimm et al. 2000 – A test for evaluating the chronic effects of plant protection products on the rove beetle Aleochara bilineata Gyll. (Coleoptera : Staphylinidae) under laboratory*
  • Chrysoperla carnea : Vogt et al. 2000 – Laboratory method to test effects of plant protection products on larvae of Chrysoperla carnea (Neuroptera: Chrysopidae)*
  • OECD 226 : Preadory mites reproduction test in soil (Hypoaspis aculeifer)*
  • Pardosa sp.: Heimbach et al. 2000 – A method for testing effects of plant protection products on spiders of the genus Pardosa (Araneae, Lycosidae) under Laboratory conditions*
  • Total fauna : ACTA – Impact des produits phytosanitaires sur la faune auxiliaire, experimental methodology in orchards (1994)
  • Typhlodromus pyri : Blümel et al. 2000 – Laboratory residual contact test with the predatory mite Typhlodromus pyri Scheuten (Acari : Phytoseiidae) for regulatory testing of plant protection products
  • Typhlodromus pyri & Kampimodromus aberrans : *
    • Blümel et al. 2000 – Laboratory residual contact test with the predatory mite Typhlodromus pyri Scheuten (Acari : Phytoseiidae) for regulatory testing of plant protection products;
    • CEB N°192 : Méthode d’étude des effets non intentionnels sur les Phytoseiidae (=Typhlodromes) en arboriculture ;
    • CEB N°167 : Méthode pour l’évaluation des effets non intentionnels a moyen terme sur les phytoseiidae (=Typhlodromes) de la vigne des préparations PHYTOPHARMACEUTIQUES utilisées en traitement des parties aériennes

All our studies can be conducted in compliance with « Good Laboratory Practice »

RRCo aims to offer terrestrial ecotoxicological tests according to the OECD Guidelines or ISO norms.

For organic substance, RRCo offers to develop and validate analytical methods allowing test item monitoring during the various phase of test.
* Tests in partnership with ENIGMA

We can test the different species listed here below:

Adalia bipunctata, Amblydromalus limonicus, Amblyseius andersoni, Amblyseius californicus, Amblyseius cucumeris, Amblyseius degenerans, Amblyseius swirskii, Anagyrus fusciventris, Anagyrus pseudococci, Aphdius ervi, Aphelinus abdominalis, Aphidius colemani, Aphidius matricariae, Aphidoletes aphidimyza, Aphytis melinus, Atheta coriaria, Chilocurus nigritus, Coccinella septempunctata, Coccophagus sp., Cryptolaemus montrouzieri, Dacnusa sibirica, Delphastus catalinae, Diglyphus isaea, Encarsia citrina, Encarsia forsoma, Eretmocerus eremicus, Exochomus quadripustulatus, Feltiella acarisuga, Forficula auricularia, Leptomastidea abnormis, Leptomastix dactylopii, Leptomastix epona, Macrolophus, pygmaeus, Metaphycus flavus, Metaphycus helvolus, Microterys flavus, Neoseiulus californicus, Orius laevigatus, Phytoseiulus persimilis, Rodolia cardinalis, Sphaerophoria rueppellii

 

Environmental fate
  • OECD 301 Ready Biodegradability
  • OECD 302 A Inherent Biodegradability: Modified SCAS Test
  • OECD 302 B Inherent Biodegradability: Zahn-Wellens/ EVPA Test
  • OECD 305  Bioconcentration: Flow-through Fish Test
  • OECD 306  Biodegradability in Seawater
  • OECD 307  Aerobic and Anaerobic Transformation in Soil
  • OECD 308  Aerobic and Anaerobic Transformation in Aquatic Sediment
  • OECD 310  Ready Biodegradability – CO2 in sealed vessels (Headspace Test)
  • OECD 311 Anaerobic Biodegradability of Organic Compounds in Digested Sludge: by Measurement of Gas Production
  • OECD 312 Leaching in Soil Columns
  • OECD 314 Simulation Tests to Assess the Biodegradability of Chemicals Discharged in Wastewater

Our studies can be conducted in compliance with Good Laboratory Practice (GLP)

RRCo offers aquatic ecotoxicology tests according to the OECD guidelines or ISO norms.

In case your product is an effluent, a pure substance, a multi-constitute, a UVCB or with specific physico-chemical properties or toxicological (volatility, insolubility, toxicity tier 2), RRCo offers to adapt tests to your regulatory needs.

In case your substance is organic, RRCo offers to develop and validate analytical method monitoring the test item along the test.

Analytical support

For organic pure substance or multi-constitute

RRCo offers analytical method development and validation in compliance with GLP making it possible the substance in regulatory matrices (water, soil, sediment…).

 

Any doubt on your substance?

You plan to carry out test outside the regulatory framework

RRCo offers tailor-made test answering to your requirement

H14 or HP14 criteria

As part of waste or marine/continental sediments characterisation, RRCo offers to carry out tests that determine the ecotoxicity of your samples.

Leaching eluates studies Species Norms
 Inhibition of the mobility of Crustacea Daphnia magna NF EN ISO 6341 
 Inhibitory effect of water samples on the light emission of Vibrio f. Vibrio Fischeri NF EN ISO 11348-3
 Fresh water algal growth inhibition test Pseudokirchneriella subcapitata ISO 8692
– Growth inhibition test brachionus calyciflorus NF ISO 20666
– Lethality and reproduction test on crustacean Ceriodaphnia dubia NF ISO 20665
Gross sample studies
– Emergence inhibition and seed growth tests Lettuce, oat, rape… ISO 11269-2
– Earthworm mortality test Eisenia fetida ISO 11268-1

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